MICROPROPAGATION OF MEDICINAL PLANTS II. WEDELIA CHINENSIS (OSBECK) MERR.

Methods for in vitro micropropagation of Wedelia chinensis by indirect organogenesis via callus were investigated by optimizing the concentrations and combinations of different phytohormones in MS medium. Callus was induced from the leaf explants in MS medium containing BAP and NAA. Callus proliferation with development of associated nodular structures was achieved on subculturing in MS medium fortified with BAP and L-glutamine. Caulogenesis was recorded after the subculture of the nodular light green friable calli on MS basal medium in 2 weeks time. Rhizogenesis was observed 7 days after reinoculation of the shoots in MS basal medium.
No detectable differences were recorded between the donor and in vitro generated plants with regard to biochemical and cytological parameters.