Mesenchymal stromal cells therapy alone does not lead to the complete restoration of the skin parameters in diabetic foot patients within a 3-year follow-up period

Mesenchymal stromal cells therapy alone does not lead to the complete restoration of the skin parameters in diabetic foot patients within a 3-year follow-up period Nadezhda V. Maksimova Department of Endocrinology, Sechenov First Moscow State Medical University (Sechenov University), Russia http://orcid.org/0000-0003-4276-0625 Anna V. Michenko Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology, Moscow, Russia Olga A. Krasilnikova Department of Regenerative Technologies and Biofabrication, National Medical Research Radiological Center, Obninsk, Russia http://orcid.org/0000-0002-8069-9952 Ilya D. Klabukov Department of Regenerative Technologies and Biofabrication, National Medical Research Radiological Center, Obninsk, Russia http://orcid.org/0000-0002-2888-7999 Igor Yu. Gadaev Chair of Hospital Therapy №1, Sechenov First Moscow State Medical University (Sechenov University), Russia http://orcid.org/0000-0002-2782-4179 Michael E. Krasheninnikov Research and Educational Resource Center for Cellular Technologies, Peoples’ Friendship University of Russia, Moscow, Russia http://orcid.org/0000-0002-3574-4013 Pavel A. Belkov Company ANTA-Med, Moscow, Russia Aleksey V. Lyundup Research and Educational Resource Center for Cellular Technologies, Peoples’ Friendship University of Russia, Moscow, Russia http://orcid.org/0000-0002-0102-5491

Introduction: Mesenchymal stromal cells (MSCs) administration is an effective option for the treatment of diabetic foot ulcers (DFUs). However, to date, studies assessing long-term outcomes and evaluating skin parameters after cell-based therapy are lacking. We presented the clinical outcomes of 3 patients, treated for DFUs with the bone marrow MSCs 3 years earlier. Methods: Ultrasound examination was used to compare collagen density and epidermal thickness in areas of healed ulcers in comparison with non-affected skin used as a control. Ultrasound and dermatoscopy were used to exclude neoplasm formation, to assess scar contracture and wound recurrence. Results: In all patients, no ulcer recurrence was detected, which was lower than the expected 60% rate of re-ulceration in diabetic patients in a 3-year period (OD [odds ratio] = 0.095, P = 0.12). No neoplasm formation, no contracture of hypertrophic scar, and adjacent tissue were registered. Collagen ultrasound density was decreased by 57% (P = 0.053) and epidermal thickness was increased by 72% (P = 0.01) in the area of healed ulcers in all patients. Conclusion: MSCs therapy alone did not result in the complete restoration of the skin parameters within a 3-year period. MSCs may represent important adjuvant to the therapy, however, other novel approaches are required to achieve better results.
09 25 2021 09 25 2021 1 10.34172/crossmark_policy bi.tbzmed.ac.ir false Tabriz University of Medical Sciences Tabriz University of Medical Sciences 2020-05-26 2020-07-29 2021-09-25 10.34172/bi.2021.22167 20210930144027 https://bi.tbzmed.ac.ir/Inpress/bi-22167 https://bi.tbzmed.ac.ir/Inpress/bi-22167.pdf https://bi.tbzmed.ac.ir/Inpress/bi-22167.pdf https://bi.tbzmed.ac.ir/Inpress/bi-22167.pdf https://bi.tbzmed.ac.ir/Inpress/bi-22167.pdf https://bi.tbzmed.ac.ir/Inpress/bi-22167.pdf